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Marchler-Bauer A, et al. Comparisons of the genomes of new giant phages isolated from environmental Pseudomonas aeruginosa strains of different regions. The phage was amplified as previously described 13 , and DNA was extracted according to reference Aziz RK, et al. A CoreGenes analysis 14 was performed and revealed 63 proteins showing similarity. The host of this phage is Pseudomonas putida variant Manila, a common rhizosphere bacterium.

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Mattheusa A. The Pfam protein families database. Uniquely structured tail fibers are visible coiling along the tail sheath. The giant bacteriophage Lu11 was isolated in from a soil sample taken under a mango tree in the Philippines 5. Krylovd A. Abstract The complete genome sequence of the tiant Pseudomonas phage Lu11 was determined, comparing and Sanger sequencing. The phage was amplified as previously described 13and DNA was extracted according to reference Please review our privacy policy.

Burkal’tseva MV, et al. Adriaenssensa, b, c W. However, the genome data presented here shows that Lu11 is an orphan species within the Myoviridae. Nucleotide sequence accession number. This research was performed by members of the PhageBiotics research community reference numbers WO.

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Aziz RK, et al. Only eight predicted genes coding for parts of the virion particle were found, including tail tube and sheath, baseplate wedge, and tail fibers. Bailey TL, et al. No DNA homology was observed with any phage. The complete genome sequence of the giant Pseudomonas phage Lu11 was determined, gaint and Sanger sequencing.

Comparisons of the genomes of new giant phages isolated from environmental Pseudomonas aeruginosa strains of different regions. Received Mar 13; Accepted Mar Comparison of new giant bacteriophages OBP and Lu11 of soil pseudomonads with bacteriophages of phiKZ-supergroup of Pseudomonas aeruginosa.

Punta M, et al. Author information Article notes Copyright and License information Disclaimer.

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Three putative early host-dependent promoters were identified, all located upstream from small hypothetical proteins. No discrepancies between the and Sanger data were observed.

Briefly, Lu11 belongs to the Myoviridae family, with an icosahedral head of nm and a contractile tail nm in length and 20 nm gint.

Sambrook J, Russell D. Krylov VN, et al.

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Kropinskie and R. No tRNAs were discovered. This article has been cited by other articles in PMC. The host of this phage is Pseudomonas putida variant Manila, a common rhizosphere bacterium. Laslett D, Canback B.

Address correspondence to R. National Center for Biotechnology InformationU. As such, Lu11 can be considered phylogenetically unique within the Myoviridae and indicates the diversity of the giant phages within this family.